1. A comparative evaluation of avidin-biotin ELISA and micro SNT for detection of antibodies to infectious bovine rhinotracheitis in cattle population of Odisha, India - Priyaranjan Das, Nihar Nalini Mohanty, S. Ranganatha, Siddharth Ranabijuli, Laxmi Narayan Sarangi and Hemant Kumar Panda
Veterinary World, 7(8): 548-552

   doi: 10.14202/vetworld.2014.548-552

Priyaranjan Das: Orissa Biological Products Institute (SatelliteUnit), Berhampur, Odisha, India; drpriya.vety@gmail.com

Nihar Nalini Mohanty: Clinical Bacteriology Laboratory, Indian Veterinary Research Institute, Mukteswar, Uttarakhand, India;nihar.mohanty13@gmail.com

S. Ranganatha: National Institute of Veterinary Epidemiology and Disease Informatics, Hebbal, Bengaluru-560024, Karnataka, India;srangavetco@gmail.com

Siddharth Ranabijuli: Krishi Vigyan Kendra, Sakhigopal, Odisha, India; drsidhu.vet@gmail.com

Laxmi Narayan Sarangi: Regional Medical Research Centre, Bhubaneswar, Odisha, India; laxmisarangi@gmail.com

Hemant Kumar Panda: Department of Veterinary Microbiology, Veterinary College, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India; drpanda.hk@gmail.com

Received: 09-04-2014, Revised: 17-06-2014, Accepted: 24-06-2014, Published online: 02-08-2014

Corresponding author: Nihar Nalini Mohanty, email: nihar.mohanty13@gmail.com

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Abstract

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Aim: The present study was undertaken to serologically detect Infectious Bovine Rhinotracheitis (IBR) in the cattle population of Odisha, India using micro-Serum neutralization test (micro SNT) and Avidin-Biotin Enzyme linked immuno sorbent assay (AB ELISA) and finding out their comparative efficacy to serve as a suitable diagnostic tool in field condition.
Materials and Methods: The study was carried out using serum samples (n=180) collected randomly from cattle populations of nine districts of Odisha. Similarly vaginal swabs (n=26) from cattle having history of repeat breeding, abortion, vulvo-vaginitis and nasal swabs (n=8) from calves with respiratory symptoms and nasal discharge were collected aseptically, to ascertain the circulation of virus among the cattle population.
Results: Virus isolation by cell culture and subsequent confirmation by polymerase chain reaction confirmed four isolates. Screening of serum samples revealed 9.44% and 12.22% samples positive for IBR antibodies in micro SNT and AB ELISA respectively. The sensitivity and specificity of AB ELISA test was found to be 88.23% and 95.70% respectively taking micro SNT as gold standard and the kappa value between the two tests was 0.75.
Conclusion: Screening of serum samples revealed 9.44% and 12.22% samples positive for IBR antibodies in micro SNT and AB ELISA respectively, thus highlighting the circulation of virus among the livestock population of Odisha and that AB ELISA could be more efficiently applied for the sero-diagnosis of IBR virus infections at field conditions, with demand for more study on faster, efficient and large scale screening of the infected animals.
Keywords: avidin-biotin ELISA, bovine herpes virus -1, cattle, infectious bovine rhinotracheitis, isolation, serum neutralization test, Odisha.

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References

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1. MacLachlan, N. J and Dubovi, E. J. (2011) Fenner's Veterinary Virology. 4th Edn. Academic Press, London. p180.

2. Biswas S., Bandyopadhyay S., Dimri U and Patra P.H. (2013) Bovine herpesvirus-1(BHV-1) a re-emerging concern in livestock: a revisit to its biology, epidemiology, diagnosis, and prophylaxis. Vet Qurt., 33(2):68-81. http://dx.doi.org/10.1080/01652176.2013.799301 PMid:23802762

3. Bandyopadhaya, S., Chakraborty, D., Sarkar, T., Pal, B., Sasmal, D., Biswas, T.K., Ghosh, M.K. and Sarkar, M. (2009) A serological survey of antibodies against bovine herpes virus-1 in yak (Poephagus grunniens) in Arunachal Pradesh in India. Rev. sci. tech. Off. int. Epiz., 28 (3):1045- 1050.

4. Ganguly, S., Mukhopadhya, S. K. and Paul, I. (2008) Studies on seroprevalence of infectious bovine rhinotracheitis in cattle population of West Bengal. Indian J. Comp.Microbiol. Immunol. Infect. Dis., 29(1-2): 12-16.

5. Kiran K. K., Ravi, P. and Prabhudas, K. (2005) Infectious bovine rhinotracheitis- National survey of IBR antibodies by AB-ELISA kit. Annual Report of Project Directorate on Animal Disease Monitoring and Surveillance. ICAR, Bangalore. p7-10.

6. Reed, L. J. and Muench, H. (1938) A simple method of estimating fifty per cent endpoints. Amer. J. Hyg. 27: 493.

7. Tongaonkar, S. S., Singh, B. K. and Rama, K. (1986) Comparative prevalence of the infectious bovine rhinotracheitis, Bluetongue, Chlamydia and Brucella antibodies in dairy animals. Indian J. Comp. Microbiol. Immunol. Infect. Dis.,7: 139-143.

8. Suresh, K. B., Sudharshana, K. J and Rajasekhar, M. (1999). Seroprevalence of infectious bovine rhinotracheitis in India. Indian Vet. J., 76: 5-9.

9. Gungor, A. B and Ozkul, A. (2007). Dynamics of natural bovine herpesvirus-1 (BHV-1) infection in a dairy herd. Trop Anim Health Prod., 39: 13–20. http://dx.doi.org/10.1007/s11250-006-4411-7 PMid:17941483

10. Chandranaik, B. M., Chethana, S., Kumar, S and Renukaprasad, C. (2010) Isolation of BHV-1 from bovine semen and application of real-time PCR for diagnosis of IBR/IPV from clinical samples. Vet. Arhiv., 80: 467-475.

11. Nisavic, J., Milic, N., Aleksandra, K and Tanja, J. (2010) The application of polymerase chain reaction in detection of bovine herpes virus 1 in clinical samples. Acta Vet., 60 (1): 39-48. http://dx.doi.org/10.2298/AVB1001039N

12. Singh, B. K., Sreenivasan, M. A., Tongaonkar, S. S., Mukherjee, F and Shrivastava, P. K. (1989) Isolation of infectious bovine rhinotracheitis from two out breaks of respiratory tract infections in cattle. Indian J. Anim. Sci., 59 (6): 644-646.

13. Ross, C., Riquelme, M.E., Ohman, F. K. and Belak, S. (1999) Improved detection of five closely related ruminant alphaherpesviruses by specific amplification of viral genome sequences. J. Virol. Methods., 83: 55–65. http://dx.doi.org/10.1016/S0166-0934(99)00103-2

14. Smits C.B., Van Maanen C., Glas R.D, De Gee A.L., Dijkstrab T., Van Oirschot J.T. and Rijisewijk F.A. (2000). Comparison of three polymerase chain reaction methods for routine detection of bovine herpesvirus 1 DNA in fresh bull semen. J. Virol. Methods, 85, 65–73. http://dx.doi.org/10.1016/S0166-0934(99)00153-6

15. Anita, D., Anita, A. and Savuta, G.(2010). Detection of Bovine Herpesvirus Type 1 by PCR Assay. Bulletin UASVM, Veterinary Medicine., 67(2):23-27.

16. Kumar, M., Manohar, M. and Nandi, S.(2011). A rapid PCR for detection of BHV-1 genomic DNA in semen samples. Indian Vet. J., 88(6):14-16.

17. Bauer, K., Gerbermann, J. H., Schmittodiet, E. and Winteroll, G. (1980) Serological investigation into the distribution of IBR/IPV in cattle in Bavaria. Tierarztl Umschau, 35(9): 599- 600.

18. Wiyong, A. (1993) Study on the prevalence of antibody to infectious rhinotracheitis virus in sentinel Bali cattle and their calves in Lampung. Penyakit-Hewan, 25: 7-10.

19. Ceylan, A., Tan, M.T., Dagalp,S. B., Yildirim, Y. and Serin, I. (2009) Seroprevalence of IBR/IPV in dairy cows with reproductive problems. Indian Vet. J., 86 (1): 7-9.

20. Nandi, S., Kumar, M., Yadav, V and Chander, V. (2011) Serological evidences of bovine herpesvirus-1 infection in bovines of organized farms in India. Transbound Emerg Dis., 58: 105–109. http://dx.doi.org/10.1111/j.1865-1682.2010.01185.x PMid:21156033

21. Pandey, A. B., Mehrotra, M. L., Verma, R. P and Pati, U. S. (2000) Investigation of an outbreak of infectious pustular balanoposthitis in breeding bulls. Indian J. Vet. Res., 9: 27-37.

22. Renukaradhya, G. J., Rajasekhar, M. and Raghavan, R. (1996) Prevalence of infectious bovine rhinotracheitis in Southern India. Rev. Sci. Tech. Off. Int. Epiz., 15: 1021-1028.

23. Singh, A., Sinha, B. K. and Jayachandran, C. (2010) Seroprevalence of IBR in normal and diseased bovines. Indian Vet. J., 87 (4): 393-394.

24. Trangadia, B. J., Rana, S. K., Nagmani, K. and Srinivasan, V. A. (2012) Serological Investigation of Bovine Brucellosis, Johne's disease and Infectious Bovine Rhinotracheitis in Two States of India. J. Adv. Vet. Anim. Res., 2: 38-41.

25. Sharma, M., Katoch, R. C. and Dhar, P. (2006) Seroprevalance of IBR among cattle in Himachal Pradesh. Indian Vet. J., 83: 1-3.

26. Shome, B. R., Rajeswari, S. and Shrivastava, N. (1997) Sero- prevalence of antibodies to BHV-1 in cattle of Andaman. Indian Vet. J., 74: 734-736.

27. Dhand, N. K., Singh, G., Sharma, D. R. and Sandhu, K. S. (2002) Seroprevalence of IBR in Punjab. Indian J. Anim. Sci., 72: 850-852.

28. Boelaert, F., Biront, F., Soumare, B., Dispas, M., Vanopdenbosch, E., Vermeersch, J. P., Raskin, A., Dufey, J., Berkvens, D. (2000) Prevalence of bovine herpesvirus-1 in the Belgian cattle population. Prev Vet Med., 45: 285-295. http://dx.doi.org/10.1016/S0167-5877(00)00128-8