Research (Published online: 08-11-2014)
5. Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region - Jobin Thomas, Mithilesh Singh, T. K. Goswami, Suman Verma and Surendra Kumar Badasara
Veterinary World, 7(10): 929-932
doi: 10.14202/vetworld.2014.929-932
Aim: The aim of this study was to screen the suspected samples by polymerase chain reaction (PCR) and epidemiological analysis of positive cases of canine parvovirus type2.
Materials and Methods: Fecal samples were collected from dogs suspected for canine parvovirus type 2 (CPV-2) and viral DNA was extracted. Primers were designed, and PCR was done with all extracted DNA samples. Age, sex and breed wise distribution of positive cases were analyzed.
Results: Out of a total 44 collected fecal samples, 23 were found to be positive for CPV-2 by developed PCR. The disease was found to be more common in Labrador male pups of 3-6 months of age. The percentage of positive cases in vaccinated dogs was found to be around 17.4%.
Conclusion: Almost half (52.3%) of total collected samples were found to be positive by PCR. However, number of field samples are needed to further validate this test and additionally sequence analysis needs to be done to ensure the prevalent field strain of CPV-2.
Keywords: Canine parvovirus type 2, fecal sample, polymerase chain reaction.
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