Audience: Veterinary World readers represent education, industry and government, including research, teaching, administration, veterinary medicine and technical services in more than 150 countries. Veterinary World is of interest to those in veterinary medicine, infectious diseases, public health, parasitology, food science, epidemiology, immunology, virology, bacteriology, nutrition, pathology, physiology, gynaecology, wildlife.
Sunday, 4 June 2017
Prevalence of methicillin-resistant Staphylococcus aureus skin and nasal carriage isolates from bovines and its antibiogram
Research (Published online: 04-06-2017)
4. Prevalence of methicillin-resistant Staphylococcus aureus skin and nasal carriage isolates from bovines and its antibiogram
Alok Kumar, Purushottam Kaushik, Anjay, Pankaj Kumar and Manoj Kumar
Veterinary World, 10(6): 593-597
Aim:This study was conducted to determine the
prevalence of methicillin-resistant Staphylococcus aureus(MRSA) in cattle and buffalo and to
study their antibiotic resistance pattern.
and Methods:A total
of 136 samples (skin and nasal swab) from cattle and buffalo were collected.
MRSA was identified by conventional bacterial
culture techniques which were further confirmed by amplification ofS.
aureus-specific 16S rRNA by polymerase chain reaction (PCR). The
isolates were further analyzed for the presence of mecA gene by PCR. The antimicrobial susceptibility profiling was performed by disc diffusion
Results:The prevalence of MRSA in the current study
was 28.57% and 34.28% in cattle nasal and skin swab, respectively, with an
overall prevalence of
31.43% MRSA among cattle. Buffalo nasal and skin sample showed MRSA prevalence
of 54.55% and 39.4%, respectively, with 46.9% overall prevalence. PCR
could detect mecA gene in 36.4% and 58% MRSA isolates from cattle and buffalo,
respectively. Antimicrobial susceptibility test found MRSA resistant to
penicillin and oxytetracycline (88% each), cefoxitin (75%), cotrimoxazole
(62%), and amoxyclav (50%). 100% sensitivity was observed against
chloramphenicol, and gentamicin. Three (16.7%) MRSA isolates from buffalo were
found resistant to vancomycin.
Conclusion:Cattle and buffalo were identified as a
potential carrier of MRSA in Bihar (India).
The isolation of
vancomycinresistant S. aureus (VRSA) in the
current study indicates the emergence of VRSA in animal population which may be
transmitted to the human beings working in close contact to the