Audience: Veterinary World readers represent education, industry and government, including research, teaching, administration, veterinary medicine and technical services in more than 150 countries. Veterinary World is of interest to those in veterinary medicine, infectious diseases, public health, parasitology, food science, epidemiology, immunology, virology, bacteriology, nutrition, pathology, physiology, gynaecology, wildlife.
Wednesday, 2 August 2017
Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks
Research (Published online: 02-08-2017)
2. Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks
Hazem Mohammed Ibrahim, Rafik Hamed Sayed, Wafaa Ragab Abdel-Aziz and Rafik Tawfik Soliman
Veterinary World, 10(8): 848-853
Aim: The present work aimed to develop lateral flow immunochromatographic strip (ICS) test for detection of Salmonella Enteritidis (SE) specific antibodies in chicken sera.
Materials and Methods: A rapid lateral flow immunochromatographic test (LFIT) has been developed, in which SE Group D antigen labeled with the gold chloride molecules laid on the conjugate pad. Staphylococcus aureus protein A was used as capture antibody at the test line (T) of a nitrocellulose (NC) membrane and anti-SE antigen-specific rabbit antibodies were used as capture antibody at the control line (C) of the NC strip in the lateral flow layout device.
Results: Using the developed LFIT, the minimal amount of SE-specific antibodies that can be detected in chicken serum sample was 1427 enzyme-linked immunosorbent assay (ELISA) unit/100 μl that was equal to 0.1 μg (Ab)/100 μl sample. 100 suspected serum samples collected from a poultry flock were tested with the prepared SE-LFIT kits and the locally prepared stained Salmonella antigen, and the results were compared with those obtained from examination of these samples with Salmonella Group D antibody ELISA kit as the gold standard test. The sensitivity, specificity, and accuracy of the prepared SE-LFIT antigen kits were 94.4%, 90%, and 94%, respectively, while those obtained with stained Salmonella antigen were 88.8%, 90%, and 89%, respectively.
Conclusion: The developed test is a simple field rapid test of high sensitivity, specificity, and accuracy that can improve and facilitates rapid field surveillance of salmonellosis among chickens.