Influence of post hatch dietary supplementation of fat on performance, carcass cuts and biochemical profile in Ven Cobb broiler

Research (Published online: 16-02-2015)

10. Influence of post hatch dietary supplementation of fat on performance, carcass cuts and biochemical profile in Ven Cobb broiler -Komal Prasad Rai, M. K. Gendley, S. P. Tiwari, Tarini Sahu and Surendra Kumar Naik

Veterinary World, 8(2): 187-191

   doi: 10.14202/vetworld.2015.187-191

Citation: Rai KP, Gendley MK, Tiwari SP, Sahu T, Naik SK (2015) Influence of post hatch dietary supplementation of fat on performance, carcass cuts and biochemical profile in Ven Cobb broiler, Veterinary World 8(2):187-191.

---

Abstract

---

Aim: The present experiment was conducted to study the effect of post hatch dietary fat supplementation on performance of broiler chicken.

Materials and Methods: A total of 120 day-old Ven Cobb broiler chicks were randomly assigned to 4 treatment groups of 30 chicks in each (three replicates of 10 birds/treatment). The trial lasted for 35 days. The experimental design was a completely randomized design. Four types of diet were formulated for 1st week: T1, T2, T3 and T4 contained control diet with no added fat, 2.5, 5 and 7.5% fat, respectively. After 1st week post-hatch period chicks were fed ad libitum with the normal basal diet as per Bureau of Indian Standard recommendations till completion of the experiment (8-35 days).

Results: Significantly higher (p<0.05) body weight and improved feed conversion ratio (FCR) was recorded in birds fed 5% dietary fat at the end of the experiment whereas, feed intake was not significantly affected. Significantly (p<0.05) higher dressed weight was observed due to 5% fat supplementation than other groups whereas, it was not significant for other carcass cuts. No significant differences were observed in moisture, protein and lipid content of breast and thigh muscle of broiler due to supplemented fat whereas, 2.5% dietary fat significantly (p<0.05) increase the serum HI titer on day 28th. In biochemical profile, higher serum albumin (g/dl) was recorded due to 5% fat supplementation whereas other biochemical components did not show any significance difference among treatments.

Conclusion: It may be concluded that supplementation of fat in broilers diet improves the overall FCR, dressing percentage and gain more body weight.

Keywords: biochemical profile, broiler, carcass cuts, fat, performance, post hatch.

Chromosomal profile of indigenous pig (Sus scrofa)

Research (Published online: 16-02-2015)

9. Chromosomal profile of indigenous pig (Sus scrofa) - P. Guru Vishnu, B. Punyakumari, B. Ekambaram, M. Gnana Prakash and B. V. Subramanyam

Veterinary World, 8(2): 183-186

   doi: 10.14202/vetworld.2015.183-186

Citation: Guruvishnu P, Punyakumari B, Ekambaram B, Prakash MG, Subramanyam BV (2015) Chromosomal profile of indigenous pig (Sus scrofa),Veterinary World, 8(2) 183-186.

---

Abstract

---

Aim: The objective of this study was to investigate the chromosomal profile of indigenous pigs by computing morphometric measurements.

Materials and Methods: A cytogenetic study was carried out in 60 indigenous pigs to analyze the chromosomal profile by employing the short term peripheral blood lymphocyte culture technique.

Results: The modal chromosome number (2n) in indigenous pigs was found to be 38 and a fundamental number of 64 as in the exotic. First chromosome was the longest pair, and thirteenth pair was the second largest while Y-chromosome was the smallest in the karyotype of the pig. The mean relative length, arm ratio, centromeric indices and morphological indices of chromosomes varied from 1.99±0.01 to 11.23±0.09, 1.04±0.05 to 2.95±0.02, 0.51±0.14 to 0.75±0.09 and 2.08±0.07 to 8.08±0.15%, respectively in indigenous pigs. Sex had no significant effect (p>0.05) on all the morphometric measurements studied.

Conclusion: The present study revealed that among autosomes first five pairs were sub metacentric, next two pairs were sub telocentric (6-7), subsequent five pairs were metacentric (8-12) and remaining six pairs were telocentric (13-18), while both allosomes were metacentric. The chromosomal number, morphology and various morphometric measurements of the chromosomes of the indigenous pigs were almost similar to those established breeds reported in the literature.

Keywords: chromosomal profile, indigenous pigs, idiogram, morphometric measurements.

Evaluation of egg production after adoption of biosecurity strategies by backyard poultry farmers in West Bengal

Research (Published online: 16-02-2015)

8. Evaluation of egg production after adoption of biosecurity strategies by backyard poultry farmers in West Bengal - I. Samanta, S. N. Joardar, D. Ganguli, P. K. Das and U. Sarkar

Veterinary World, 8(2): 177-182

   doi: 10.14202/vetworld.2015.177-182

Citation: Samanta I, Joardar SN, Ganguli D, Das PK, Sarkar U (2015) Evaluation of egg production after adoption of biosecurity strategies by backyard poultry farmers in West Bengal, Veterinary World, 8(2):177-182.

---

Abstract

---

Aim: On the basis of identified source of major bacterial infections at four agro-climatic zones in West Bengal the cost-effective biosecurity strategy was formulated for backyard poultry farmers. The aim of the present study was to assess the adoption. So, the study was aimed to detect the adoption level of the formulated biosecurity strategy to mitigate the Salmonella and Escherichia colicontamination level in the sources and its correlation with egg production in West Bengal.

Materials and Methods: A questionnaire was prepared querying regarding the biosecurity measures presently followed by the farmers, if any and egg production of their birds. Subsequent to the interview the formulated biosecurity strategy was conveyed. After 3 months, the interview with the same questionnaire was conducted to the same farmers to detect their adoption level.

Results: The change in practices were noted in certain parameters which differs significantly (p<0.01 or p<0.05). As a consequence, the average egg production/flock was increased in 3 months after adoption of the strategy (618.2±37.77/flock) in comparison to last 3 months average before adoption of the strategy (495.3±30.00/flock) which also differs significantly (p<0.01).

Conclusion: The present study detected the implementation of the biosecurity strategy in backyard poultry farming in West Bengal can substantially benefit the farmers in terms of increased egg production.

Keywords: adoption, backyard poultry, biosecurity strategy, egg production, West Bengal.

Fungal biological control agents for integrated management of Culicoides spp. (Diptera: Ceratopogonidae) of livestock

Research (Published online: 10-02-2015)

5. Fungal biological control agents for integrated management of Culicoides spp. (Diptera: Ceratopogonidae) of livestock - B. W. Narladkar, P. R. Shivpuje and P. C. Harke

Veterinary World, 8(2): 156-163

   doi: 10.14202/vetworld.2015.156-163

Abstract

---

Aim: Entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana had wide host range against insects and hence these are being exploited as fungal bio-pesticide on a large scale. Both fungi are proved pesticides against many crop pests and farmers are well acquainted with their use on the field. Thus, research was aimed to explore the potency of these fungal spores against larval and adultCulicoides midges, a pest of livestock.

Materials and Methods: In-vitro testing of both fungal biological control agents was undertaken in Petri dishes against field collectedCulicoides larvae, while in plastic beakers against field collected blood-engorged female Culicoides midges. In-vivo testing was undertaken by spraying requisite concentration of fungal spores on the drainage channel against larvae and resting sites of adultCulicoides midges in the cattle shed. Lethal concentration 50 (LC50) values and regression equations were drawn by following probit analysis using SPSS statistical computerized program.

Results: The results of this study revealed LC50 values of 2692 mg and 3837 mg (108 cfu/g) for B. bassiana and M. anisopliae, respectively, against Culicoides spp. larvae. Death of Culicoides larvae due to B. bassiana showed greenish coloration in the middle of the body with head and tail showed intense blackish changes, while infection of M. anisopliae resulted in death of Culicoides larvae with greenish and blackish coloration of body along with total destruction, followed by desquamation of intestinal channel. The death of adultCulicoides midges were caused by both the fungi and after death growth of fungus were very well observed on the dead cadavers proving the efficacy of the fungus.

Conclusion: Preliminary trials with both funguses (M. anisopliae, B. bassiana) showed encouraging results against larvae and adults ofCulicoides spp. Hence, it was ascertained that, these two fungal molecules can form a part of biological control and alternative to chemical control and, therefore, can be inducted in integrated management programs.

Keywords: biocontrol agents, Beauveria bassiana, Culicoides spp, integrated pest management, Metarhizium anisopliae.

Construction and characterization of recombinant human adenovirus type 5 expressing foot-and-mouth disease virus capsid proteins of Indian vaccine strain, O/IND/R2/75

Research (Published online: 10-02-2015)

4. Construction and characterization of recombinant human adenovirus type 5 expressing foot-and-mouth disease virus capsid proteins of Indian vaccine strain, O/IND/R2/75 - Ramesh Kumar, B. P. Sreenivasa and R. P. Tamilselvan

Veterinary World, 8(2): 147-155

   doi: 10.14202/vetworld.2015.147-155

Abstract

---

Aim: Generation of recombinant human adenovirus type 5 expressing foot-and-mouth disease virus (FMDV) capsid protein genes along with full-length 2B, 3B and 3Cpro and its characterization.

Materials and Methods: FMD viral RNA isolation, cDNA synthesis, and polymerase chain reaction were performed to synthesize expression cassettes (P1-2AB3BCwt and P1-2AB3BCm) followed by cloning in pShuttle-CMV vector. Chemically competent BJ5183-AD-1 cells were transformed with the recombinant pShuttle-CMV to produce recombinant adenoviral plasmids. HEK-293 cells were transfected with the recombinant adenoviral plasmids to generate recombinant adenoviruses (hAd5/P1-2AB3BCwt and hAd5/P1-2AB3BCm). Expression of the target proteins was analyzed by sandwich ELISA and indirect immunofluorescence assay. The recombinant adenoviruses were purified and concentrated by CsCl density gradient ultracentrifugation. Growth kinetics and thermostability of the recombinant adenoviruses were compared with that of non-recombinant replication-defective adenovirus (dAd5).

Results: The recombinant adenoviruses containing capsid protein genes of the FMDV O/IND/R2/75 were generated and amplified in HEK-293 cells. The titer of the recombinant adenoviruses was approximately 108, 109.5 and 1011 TCID50/ml in supernatant media, cell lysate and CsCl purified preparation, respectively. Expression of the FMDV capsid protein was detectable in sandwich ELISA and confirmed by immunofluorescence assay. Growth kinetics of the recombinant adenoviruses did not reveal a significant difference when compared with that of dAd5. A decrement of up to 10-fold at 4°C and 21-fold at 37°C was recorded in the virus titers during 60 h incubation period and found to be statistically significant (p<0.01).

Conclusion: Recombinant adenoviruses expressing capsid proteins of the FMDV O/IND/R2/75 were constructed and produced in high titers. In vitro expression of the target proteins in the adenovirus vector system was detected by sandwich ELISA and immunofluorescence assay.

Keywords: foot-and-mouth disease, growth kinetics, recombinant hAd5, thermostability, virus-like particles.

Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro

Research (Published online: 09-02-2015)

3. Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro - Gopal Puri, S. S. Chaudhary, V. K. Singh and A. K. Sharma

Veterinary World, 8(2): 143-146

   doi: 10.14202/vetworld.2015.143-146

---

Abstract

---

Aim: The aim was to assess the effects of fetal bovine serum (FBS) and estrus buffalo serum (EBS) on in vitro maturation rate of oocytes in buffalo.

Materials and Methods: Maturation rate of oocytes was assessed in two maturation media supplemented with 20% FBS and EBS. Oocytes maturation rate was evaluated on the basis of cumulus cell expansion and extrusion of polar body after 24 h of in vitro culture in CO2 incubator.

Results: The average percentage of in vitro matured oocytes in FBS was 83.80%, and EBS was 77.45%, respectively. The results revealed a significant (p<0.05) increase in maturation rate of oocytes in FBS than EBS.

Conclusion: Buffalo oocytes were better in vitro matured in FBS than EBS.

Keywords: buffalo oocytes, estrus buffalo serum, fetal bovine serum, in vitro maturation.

Effect of feeding varying levels of groundnut haulms on feed intake and growth performance in broiler chickens

Research (Published online: 09-02-2015)

2. Effect of feeding varying levels of groundnut haulms on feed intake and growth performance in broiler chickens - N. K. Ribadiya, H. H. Savsani, S. S. Patil, D. D. Garg, M. R. Gadariya, V. K. Karangiya and A. P. Gajera

Veterinary World, 8(2): 139-142

   doi: 10.14202/vetworld.2015.139-142

---

Abstract

---

Aim: This study was carried out to evaluate groundnut haulms (GNH) as alternate feed source by its incorporation and assessment in terms of feed intake and growth performance in the diets of broilers.

Materials and Methods: A total of 240 1-day-old Cobb-400 broiler chicks were randomly assigned to four dietary treatments each with three replicates (n=60). Experimental Birds in group T1 were fed with conventional feed while birds in T2, T3, T4 were fed containing 2%, 4%, and 6% of GNH replacing maize and soyabean on iso-nitrogenous basis.

Results: Feed intake increases significantly (p>0.05) with increasing level of GNH in the diets of experimental birds. Highest feed intake was recorded in T4 (6% GNH), followed by T3 (4% GNH) than T2 (2% GNH) and T1 (control). Birds fed GNH gained significantly (p<0.05) higher body weight than birds fed the control diet. Birds in T4 [6% GNH] gained highest body weight, followed by T3 (4% GNH) than T2 (2% GNH) and T1 (control). However, feed conversion ratio (FCR) remained non-significant for all treatment groups.

Conclusion: On the basis of the results of this study, it is concluded that supplementation of GNH can successfully replace costly ingredients like maize and soybean meal in the diets of broiler birds up to the level of 6 percent of concentrate mixture without any harmful effects on feed intake, growth and FCR.

Keywords: body weight, broiler chickens, feed intake, groundnut haulms

Comparison of immunochromatographic diagnostic test with heminested reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species

Research (Published online: 09-02-2015)

1. Comparison of immunochromatographic diagnostic test with heminested reverse transcriptase polymerase chain reaction for detection of rabies virus from brain samples of various species - Pranoti Sharma, C. K. Singh and Deepti Narang

Veterinary World, 8(2): 135-138

   doi: 10.14202/vetworld.2015.135-138

---

Abstract

---

Aim: Detection of rabies is a cause of serious concern in developing countries, where dearth of highly equipped laboratories and trained personnel to handle sophisticated investigations is felt. The availability of a diagnostic kit, which can be used in the field, is essential for diagnosis and control programs as well as for epidemiological surveillance of the prevalence of the disease. This study was planned to evaluate anigen rabies Ag test kit for its efficacy to be used for rapid diagnosis of rabies under field conditions. The test results were compared with hemi-nested reverse transcriptase polymerase chain reaction and with a gold standard fluorescent antibody test.

Materials and Methods: A total of 34 brain samples from different rabies suspected animals including dogs, buffaloes, cow, horse, and cat were examined in this study.

Results: Sensitivity of the kit was found to be 91.66%, specificity 100%, and accuracy was 94.11%.

Conclusion: The study implies that the immunochromatographic diagnostic test kit may be employed for diagnosis of rabies in field conditions.

Keywords: antigen, heminested, immunochromatographic, rabies

The influence of altitude and landforms on some biochemical and hematological parameters in Ouled Djellal ewes from arid area of South East Algeria

Research (Published online: 31-01-2015)

25. The influence of altitude and landforms on some biochemical and hematological parameters in Ouled Djellal ewes from arid area of South East Algeria - Mohammed Titaouine and Toufik Meziane

Veterinary World, 8(1): 130-134

   doi: 10.14202/vetworld.2015.130-134

---

Abstract

---

Aim: This study was conducted on Ouled Djellal ewes in arid area of south-east Algeria in order to reveal the influence of altitude and landforms on some hematological and biochemical parameters.

Materials and Methods: A total of 160 ewes having 3-5 years of age, multiparous, non-pregnant, non-lactating and reared in arid areas of South East Algeria were included. Blood samples were divided according to factors of altitude and landform (plain region at 150 m above sea level, tableland region at 600 m above sea level and mountain region at 1000 m above sea level). The whole blood was analyzed for hematology, and plasma samples for biochemical analysis.

Results: The study found lowest glucose concentrations were detected in tableland region at 600 m. In plain region at 150 m, ewes had a higher (p<0.01) concentration of cholesterol and triglyceride. Furthermore, a higher concentration of total proteins (p<0.01) and urea (p<0.05) were detected in plain region at 150 m. The average blood creatinine concentration in mountain ewes at 1000 m and tableland ewes at 600 m were higher (p<0.05) that in plain ewes at 150 m. The highest calcium concentration was found at the altitude of 150 m and the lowest at the altitude of 1000 m (1.12±0.35 mmol/L vs. 0.52±0.03 mmol/L). Phosphorus levels were higher at altitudes of 150 m than at the altitude of 600 m and 1000 m (0.93±0.42 mmol/L vs. 0.68±0.54 mmol/L, 0.23±0.01 mmol/L). The highest hemoglobin concentration and value of hematocrit were detected in mountain ewes at the altitude of 1000 m (120.61 g/L, 40%) and the lowest at the altitude of 150 m (73.2 g/L, 31%) (p<0.001).

Conclusion: We concluded that hematological and biochemical parameters in Ouled Djellel ewes reared in arid area may be affected by altitude and landforms.

Keywords: altitude, arid area, biochemistry, hematology, landform, Ouled Djallel ewes.

Morphological and histological identification of Paramphistomum cervi (Trematoda: Paramiphistoma) in the rumen of infected sheep

Research (Published online: 30-01-2015)

24. Morphological and histological identification of Paramphistomum cervi (Trematoda: Paramiphistoma) in the rumen of infected sheep -Vijayata Choudhary, J. J. Hasnani, Mukesh K. Khyalia, Sunanda Pandey, Vandip D. Chauhan, Suchit S. Pandya and P. V. Patel

Veterinary World, 8(1): 125-129

   doi: 10.14202/vetworld.2015.125-129

---

Abstract

---

Aim: This study was undertaken to identify Paramphistomum cervi on the basis of its morphology and histology to be the common cause of paramphistomosis in infected sheep and its differentiation from other similar Paramphistomes in Gujarat.

Materials and Methods: Adult rumen flukes were recovered from the rumen of naturally infected sheep slaughtered in various abattoirs in Gujarat. Some adult flukes were flattened and stained in Borax carmine, and some were sectioned in the median sagittal plane and histological slides of the flukes were prepared for detailed morphological and histological studies.

Result: Microscopic pictures of the parasite used in identification define the similarity in the morphology and histology of the anterior sucker, pharynx, esophagus, genital atrium, posterior sucker (acetabulum) and testes to the P. cervi.

Conclusion: It can be concluded that the most common species found in sheep infected with Paramphistomosis is P. cervi on the basis of its histo-morphological appearance in Gujarat.

Keywords: abattoirs, histology, morphology, paramphistomosis, posterior sucker, rumen fluke, sheep.