Hematobiochemical profile in Surti goats during post-partum period

Research (Published online: 12-01-2016)

4. Hematobiochemical profile in Surti goats during post-partum period - Tanvi D. Manat, Sandhya S. Chaudhary, Virendra Kumar Singh, Sanjay B. Patel and Gopal Puri

Veterinary World, 9(1): 19-24

   doi: 10.14202/vetworld.2016.19-24

Abstract

---

Aim: The study was undertaken to find out the changes in hematobiochemical profile in post-partum Surti goats.

Materials and Methods: The present study was conducted on 40 Surti goats out of which 20 goats who had undergone recent parturition acted as treatment group and 20 non-pregnant animals comprised control group. Blood samples were collected from the treatment group on 0, 7, 14, 21, 30, and 45 days post kidding and once from the control group. Blood samples were analyzed for hematological parameters such as hemoglobin (Hb), packed cell volume (PCV), total erythrocyte count (TEC), total leukocyte count (TLC), differential leukocyte count, and biochemical metabolites such as total protein (TP), albumin, globulin, total cholesterol (TC), triglycerides (TG), non-esterified fatty acid (NEFA), glucose, and urea.

Results: Hb level was the highest on the 45th day and lowest on the day of parturition. Significantly low level of Hb, PCV, and lymphocyte was found on 0 day and significantly high level was found on the 45th day. TEC was also low on the 0 day and high on the 45th day post kidding, but the difference was non-significant. Mid-sized cells and granulocyte percentage decreased significantly from 0 to 45th day post-partum. TP, albumin, and urea were found to be lowest on 0 day and highest on 45th day post-partum. Urea concentration increased parallel to TP indicating an increase in urea production with catabolism of protein. The globulin concentration also increased from 0 to 45th day post-partum, but the difference was non-significant. TC, TG, and NEFA were the highest on 0 day and lowest on the 45th day post-partum. Decrease in TC and TG from 0 to 45th day post-partum indicates that the animals utilized the lipids for the supply of energy for milk production. High level of NEFA acts as an indicator of negative energy status but in the present study, the high glucose level on 0 day

indicates that the animals were in positive energy status. The glucose concentration decreased up to 14th day post-partum and then started increasing from 21st day onward.

Conclusion: Hematobiochemical parameters are indicative of health status of animals. Significantly low values of Hb, PCV, and TLC during first 2 weeks post-partum are indicative of stress. Catabolism of protein occurred during 2 weeks post-partum, as observed from increase in urea concentration. Decrease TGs and high level of NEFA during the transition period are suggestive of utilization of lipids for the supply of energy. High glucose level on 0 day indicates that the animals were in positive energy status.

Keywords: hematobiochemical parameters, post-partum period, Surti goat.

Intrigues of biofilm: A perspective in veterinary medicine

Review (Published online: 02-01-2016)

3. Intrigues of biofilm: A perspective in veterinary medicine - Umar Faruk Abdullahi, Ephraim Igwenagu, Anas Mu’azu, Sani Aliyu and Maryam Ibrahim Umar

Veterinary World, 9(1): 12-18

   doi: 10.14202/vetworld.2016.12-18

Abstract

---

Biofilm has a tremendous impact in the field of veterinary medicine, especially the livestock industry, leading to a serious economic loss. Over the years, little attention has been given to biofilm in animals with most of the research geared toward human biofilm diseases. The greatest challenge posed by biofilm is in its incredible ability to resist most of the currently existing antibiotics. This mystery can best be demystified through understanding the mechanism of the quorum sensing which regulate the pathophysiology of biofilm. Ability of biofilm formation in a variety of inanimate surfaces such as animal food contact surfaces is responsible for a host of biofilm diseases affecting animals and umans. In this review, we highlighted some of the challenges of biofilm in livestock and food industries. Also highlighted are; mechanisms of biofilm development, best diagnostic approach and possible novel therapeutic measures needed to combat the menace of biofilm in veterinary medicine.

Keywords: antibiotic resistance, biofilm, contact surfaces, quorum sensing, veterinary medicine.

Incidence of Aeromonas spp. infection in fish and chicken meat and its related public health hazards: A review

Review (Published online: 02-01-2016)

2. Incidence of Aeromonas spp. infection in fish and chicken meat and its related public health hazards: A review - Praveen Kumar Praveen, Chanchal Debnath, Shashank Shekhar, Nirupama Dalai and Subha Ganguly

Veterinary World, 9(1): 6-11

   doi: 10.14202/vetworld.2016.6-11

Abstract

---

Aeromonas is recognized to cause a variety of diseases in man. In humans, they are associated with intestinal and extraintestinal infections. With the growing importance of Aeromonas as an emerging pathogen, it is important to combat this organism. It is indisputable that Aeromonas strains may produce many different putative virulence factors such as enterotoxins, hemolysins or cytotoxins, and antibiotic resistance against different antibiotics. The ability of these bacteria to grow competitively at 5°C may be indicative of their potential as a public health hazard. Comprehensive enteric disease surveillance strategies, prevention and education are essential for meeting the challenges in the years ahead. It is important for us to promote the value of enteric cultures when patients have a gastrointestinal illness or bloody diarrhea or when multiple cases of enteric disease occur after a common exposure. With the growing importance of Aeromonas as an emerging pathogen, it is important to combat this organism. It is indisputable that Aeromonasstrains may produce many different putative virulence factors, such as enterotoxins, hemolysins or cytotoxins. It has been established that aerolysin is a virulence factor contributing to the pathogenesis of Aeromonas hydrophila infection. Fish and chicken play an important role in the transmission of this pathogen to humans. In the present study, the high prevalence of toxin-producing strains was found among the Aeromonas isolates. The ability of these bacteria to grow competitively at 5°C may be indicative of their potential as a public health hazard. The present review was constructed with a view to highlight the zoonotic importance of Aeromonas pathogen in fish and chicken meat.

Keywords: Aeromonas, antibiotic resistance, chicken, fish, public health.

Seroepidemiology of bluetongue in South Bengal

Research (Published online: 02-01-2016)

1. Seroepidemiology of bluetongue in South Bengal - Arkendu Halder, Siddhartha N. Joardar, Devi Prasad Isore, Indranil Samanta, Panchanan Parui, Dhriti Banerjee and Chandan Lodh

Veterinary World, 9(1): 1-5

   doi: 10.14202/vetworld.2016.1-5

Abstract

---

Aim: With the aim of revealing the epidemiological intricacies of bluetongue (BT) in the southern part of West Bengal state, the present study was undertaken to assess seroprevalence of BT along with identification of the vector of the disease, i.e., Culicoides midges available in the region in their breeding season with conducive environmental factors, if any.

Materials and Methods: A total of 1509 (sheep-504, goat-1005) samples were collected from three different agroclimatic zones of South Bengal viz. new alluvial, red laterite and coastal saline. To detect anti-BT antibodies in the collected serum samples, indirect-enzyme-linked immunosorbent assay (i-ELISA) was performed. Culicoides midges were collected from those agro-climatic zones of South Bengal for species identification. The meteorological parameters, viz. temperature (maximum and minimum), rainfall and relative humidity of three agro-climatic zones of South Bengal were analyzed for the months of July to December during 2010-2013.

Results: The overall seropositivity was 33.13% and 30.24% in sheep and goat, respectively as assessed by i-ELISA. In South Bengal, the predominant species of Culicoides found were Culicoides schultzei, Culicoides palpifer and Culicoides definitus.

Conclusion: Since virus transmitting species of Culicoides midges could be detected in South Bengal, besides high seropositivity in ruminants, the possibility of circulating BT virus in South Bengal is quite imminent.

Keywords: antibodies, bluetongue, Culicoides sp., enzyme linked immunosorbent assay, South Bengal.

Can Urtica dioica supplementation attenuate mercury intoxication in Wistar rats?

Research (Published online: 30-12-2015)

15. Can Urtica dioica supplementation attenuate mercury intoxication in Wistar rats? - Wafa Siouda and Cherif Abdennour

Veterinary World, 8(12): 1458-1465

   doi: 10.14202/vetworld.2015.1458-1465

Abstract

---

Aim: The objective of this study was to investigate the possible protective role of nettle Urtica dioica (UD) against Hg-induced toxicity.

Materials and Methods: A total of 28 rats were equally divided into four groups: the control, the Hg (0.8 g HgCl2/kg in the diet), the UD (1.5 ml UD/rat by gavage), and the Hg+UD group. HgCl2 was daily dissolved in distilled water and immediately mixed with the standard diet. A solution of daily infused fresh nettle leaves in boiling water (16 g in 25 ml) was obtained and then it was administrated by gavage. Biochemical and reproductive markers, in addition to glutathione (GSH) level (liver, kidney and testis) and the histological profiles (testis and epididymis) were evaluated after 1 month exposure.

Results: Compared to the control, the levels of glucose, triglycerides, urea, creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were significantly raised in the Hg group. In the latter group, the concentrations of Mg, Fe, and Ca were significantly decreased. Besides, Hg+UD group has only showed raised AST activity and reduced Mg level. Concerning the fertility markers, Hg has provoked a significant decrease in the spermatozoa’s concentration and motility and in plasma testosterone level as well. Furthermore, hepatic, renal and testicular GSH concentrations have declined significantly in the Hg treated rat compared to the control. A remarkable enhancement of the GSH level was observed in all organs of the UD group. The histological examinations of the Hg group have revealed marked testicular degeneration of the most seminiferous tubules, and showed few sperms in the lumen of epididymis ducts. However, the Hg+UD rats have demonstrated an improved histological structure with the presence of important numbers of sperms in the lumen. In addition, a clear stabilization of organized seminiferous tubules and an increased sperms’ numbers were noted in the UD supplemented rats.

Conclusion: Nettle leaves have not only played a clear protective role during Hg intoxication, but it also enhanced hepatic, renal and testicular GSH level of Wistar rats.

Keywords: biochemical markers, fertility, glutathione, mercury, Urtica dioica, Wistar rats.

Principal component analysis of biometric traits to reveal body confirmation in local hill cattle of Himalayan state of Himachal Pradesh, India

Research (Published online: 29-12-2015)

14. Principal component analysis of biometric traits to reveal body confirmation in local hill cattle of Himalayan state of Himachal Pradesh, India - Deepak Verma, Varun Sankhyan, Sanjeet Katoch and Yash Pal Thakur

Veterinary World, 8(12): 1453-1457

   doi: 10.14202/vetworld.2015.1453-1457

Abstract

---

Aim: In the present study, biometric traits (body length [BL], heart girth [HG], paunch girth (PG), forelimb length (FLL), hind limb length (HLL), face length, forehead width, forehead length, height at hump, hump length (HL), hook to hook distance, pin to pin distance, tail length (TL), TL up to switch, horn length, horn circumference, and ear length were studied in 218 adult hill cattle of Himachal Pradesh for phenotypic characterization.

Materials and Methods: Morphological and biometrical observations were recorded on 218 hill cattle randomly selected from different districts within the breeding tract. Multivariate statistics and principal component analysis are used to account for the maximum portion of variation present in the original set of variables with a minimum number of composite variables through Statistical software, SAS 9.2.

Result: Five components were extracted which accounted for 65.9% of variance. The first component explained general body confirmation and explained 34.7% variation. It was represented by significant loading for BL, HG, PG, FLL, and HLL. Communality estimate ranged from 0.41 (HL) to 0.88 (TL). Second, third, fourth, and fifth component had a high loading for tail characteristics, horn characteristics, facial biometrics, and rear body, respectively.

Conclusion: The result of component analysis of biometric traits suggested that indigenous hill cattle of Himachal Pradesh are small and compact size cattle with a medium hump, horizontally placed short ears, and a long tail. The study also revealed that factors extracted from the present investigation could be used in breeding programs with sufficient reduction in the number of biometric traits to be recorded to explain the body confirmation.

Keywords: biometrical traits, hill cattle, phenotypic characterization, principal component analysis.

Effect of microclimate alteration on milk production and composition in Murrah buffaloes

Research (Published online: 29-12-2015)

13. Effect of microclimate alteration on milk production and composition in Murrah buffaloes - Sandeep Reddy Seerapu, Ananda Rao Kancharana, Venkata Seshaiah Chappidi and Eswara Rao Bandi

Veterinary World, 8(12): 1444-1452

   doi: 10.14202/vetworld.2015.1444-1452

Abstract

---

Aim: The aim of this study was to assess the effect of microclimate alteration on temperature-humidity index (THI), milk yield, and milk composition of Murrah buffaloes during summer for a period of 90-day from March to May-2014 at Buffalo Research Station, Venkataramannagudem, Andhra Pradesh, India.

Materials and Methods: A total of 40 lactating Murrah buffaloes were selected having similar body weight, parity, and milk yield. They were divided into four groups of 10 each. Three groups of buffaloes were provided with microclimate alteration using supplemental cooling like foggers, fans and foggers plus fans, and the fourth group (control) was without any cooling system. The daily THI was measured using dry and wet bulb thermometer. The physiological responses viz. rectal temperature, respiration rate, and pulse rate were measured by a clinical thermometer, measuring the flank movements a minute and observing the pulsation of the middle coccygeal artery at the base of tail with the help of finger. Milk samples were analyzed for chemical composition viz., fat, solids-not-fat (SNF), total solids (TS), specific gravity.

Results: In the present study, significant (p<0.001) decrease in the average THI values were observed in experimental Murrah buffalo houses of GroupII (foggers), GroupIII (fans), and GroupIV (foggers and fans) compared to GroupI (control). Significant (p<0.001) decrease in average rectal temperature (°F), respiration rate (breaths/min) and pulse rate (beats/min) values were recorded in Murrah buffaloes of Groups II, III and IV compared to Group I. Significant (p<0.001) increase in the average milk yield (kg/day) was recorded in Murrah buffaloes of Groups II, III, and IV compared with Group I. Significant (p<0.001) increase in the average milk fat, SNF, and TS percent were recorded in Murrah buffalo Groups of II, III, and IV compared with Group I.

Conclusion: Microclimate alteration by the provision of foggers and air circulators in the buffalo houses increased feed intake in buffaloes resulting increased milk production, fat and SNF yield which was due to decreased heat stress in buffaloes.

Keywords: microlimate, milk composition, milk yield, physiological parameters, temperature-humidity index.

Single nucleotide polymorphism mining and nucleotide sequence analysis of Mx1 gene in exonic regions of Japanese quail

Research (Published online: 23-12-2015)

12. Single nucleotide polymorphism mining and nucleotide sequence analysis of Mx1 gene in exonic regions of Japanese quail - Diwesh Kumar Niraj, Pushpendra Kumar, Chinmoy Mishra, Raj Narayan, Tarun Kumar Bhattacharya, Kush Shrivastava, Bharat Bhushan, Ashok Kumar Tiwari, Vishesh Saxena, Nihar Ranjan Sahoo and Deepak Sharma

Veterinary World, 8(12): 1435-1443

   doi: 10.14202/vetworld.2015.1435-1443

Abstract

---

Aim: An attempt has been made to study the Myxovirus resistant (Mx1) gene polymorphism in Japanese quail.

Materials and Methods: In the present, investigation four fragments viz. Fragment I of 185 bp (Exon 3 region), Fragment II of 148 bp (Exon 5 region), Fragment III of 161 bp (Exon 7 region), and Fragment IV of 176 bp (Exon 13 region) of Mx1 gene were amplified and screened for polymorphism by polymerase chain reaction-single-strand conformation polymorphism technique in 170 Japanese quail birds.

Results: Out of the four fragments, one fragment (Fragment II) was found to be polymorphic. Remaining three fragments (Fragment I, III, and IV) were found to be monomorphic which was confirmed by custom sequencing. Overall nucleotide sequence analysis of Mx1gene of Japanese quail showed 100% homology with common quail and more than 80% homology with reported sequence of chicken breeds.

Conclusion: The Mx1 gene is mostly conserved in Japanese quail. There is an urgent need of comprehensive analysis of other regions ofMx1 gene along with its possible association with the traits of economic importance in Japanese quail.

Keywords: Japanese quail, Mx1 gene, nucleotide sequencing, polymorphism, polymerase chain reaction-single-strand conformation polymorphism.

Dominant viral pathologies in the extensive and semi-intensive animal breeding and their treatment mode in ethno veterinary medicine in Benin

Research (Published online: 23-12-2015)

11. Dominant viral pathologies in the extensive and semi-intensive animal breeding and their treatment mode in ethno veterinary medicine in Benin - T. M. Kpodékon, C. A. Ogni, H. Dassou, T. J. Dougnon, C. Boko, G. B. Koutinhouin, J. S. E. Goussanou, A. Akoegninou and I. Youssao

Veterinary World, 8(12): 1424-1434

   doi: 10.14202/vetworld.2015.1424-1434

Abstract

---

Aim: This study aims to identify the dominant viral animal pathologies and to list the traditional recipes used by the breeders for their treatment.

Materials and Methods: The method of data collection was based on a retrospective survey. Thus, 787 breeders and agro-breeders scattered in the eight agro-ecological areas of Benin were interviewed using semi-structured questionnaires.

Results: In total, 5 pathologies were reported by breeders. Among those pathologies, foot and mouth disease was reported by all of the breeders of the southern part of Borgou compared with the other areas (p<0.05) and treated by 25 species of medicinal plants. African swine fever was the main pathology reported (22.92%) (p<0.05) in the fishery areas which is controlled by 7 medicinal plants. Pseudorinderpest was more reported (33.78%) (p<0.05) in the cotton area of central Benin and treated by 8 medicinal plants. There is also Newcastle disease that was mostly reported in the Western Atacora and treated by 32 medicinal plants as well as fowl pox which was a more reported in the lands of the bar area and the low-pressure area about 34.48% and 36.17% proportions, respectively, and treated by eight medicinal plants.

Conclusion: The breeders in Benin possess rich ethno veterinary knowledge on medicinal plants and their uses in the treatment of livestock. A total of 57 medicinal plants have been inventoried to fight against five major viral diseases as African swine fever, pseudorinderpest and foot and mouth disease. The common plants used to treat viral disease in general were Euphorbia unispina,Euphorbia poissonii, Lannea acida, and Mangifera indica. The most harvested organs on the plants reported in this survey were the barks, the leaves, and the whole plants. To better develop our indigenous resources, it would be important to expand this ethno-pharmacological investigation to other diseases category.

Keywords: Benin, ethnoveterinary survey, medicinal plants, viral diseases.

Molecular identification of Campylobacter jejuni and coli from chicken, calves and dogs to determine its potential threat on human being

Research (Published online: 18-12-2015)

10. Molecular identification of Campylobacter jejuni and coli from chicken, calves and dogs to determine its potential threat on human being - Sonuwara Begum, M. Sekar, L. Gunaseelan, Monica Gawande, G. Suganya, P. Annal Selva Malar and A.Karthikeyan

Veterinary World, 8(12): 1420-1423

   doi: 10.14202/vetworld.2015.1420-1423

Abstract

---

Aim: Campylobacter is an emerging zoonotic pathogen and one of the leading cause of foodborne infection worldwide and it has been isolated from a variety of animal species.The aim of this study was to identify Campylobacter jejuni and Campylobacter coli from dogs, calves, and poultry using polymerase chain reaction (PCR).

Methodology: A total of 104 number of samples comprising cloacal swab from poultry (38), a rectal swab from dogs (40), and calves (26) were collected for the isolation of thermophilic Campylobacters using conventional culture method. PCR was used for identification of mapA gene for C.jejuni and ceuE for C.coli.

Results: The overall presence of Campylobacter was found to be 67(64.42%) from the samples, out of which 6 isolates belongs to C. jejuni species, were 5(18.51%) from chicken and 1(4.17%) from dog was recorded and about 17 isolates belongs to C. coli species were 9(33.33%), 6 (25%), and 1(9.09%) from chicken, dog and calves was recorded.

Conclusion: Results suggested that Campylobacter reservoirs chicken, calves and pet dogs can play a role as the source of infection to human beings and PCR can be an ideal tool for molecular confirmation at the species level.

Keywords: emerging, pathogen, thermophilic Campylobacter, zoonotic.