Friday 11 March 2016

Histomorphological studies of broiler chicken fed diets supplemented with either raw or enzyme treated dandelion leaves and fenugreek seeds

Research (Published online: 12-03-2016)
8. Histomorphological studies of broiler chicken fed diets supplemented with either raw or enzyme treated dandelion leaves and fenugreek seeds - Saim Qureshi, Mohammed Tufail Banday, Irfan Shakeel, Sheikh Adil, Masood Saleem Mir, Yasir Afzal Beigh and Umar Amin
Veterinary World, 9(3): 269-275



   doi: 10.14202/vetworld.2016.269-275



Aim: Herbal plants and their derived products are extensively used particularly in many Asian, African, and other countries of the world as they are considered as ideal feed additives because of their non-residual effect and ability to influence the ecosystem of gastrointestinal microbiota in a positive way. Further, the enzymatic treatment of these herbs helps in their efficient utilization by the host. Dandelion leaves and fenugreek seeds have been reported to have positive effect in terms of improving the performance of broiler chicken, but not much literature is available regarding their effect on gut histomorphology; therefore, the present study was conducted to explore the effect of these herbs either alone or in combination with or without enzyme treatment on histomorphology of liver and small intestine of broiler chicken.
Materials and Methods: To achieve the envisaged objective, 273-day-old commercial broiler chicks were procured from a reputed source and reared together until 7 days of age. On the 7th day, the chicks were individually weighed, distributed randomly into 7 groups of 3 replicates with 13 chicks each. Birds in the control group were fed diets without additives (T1). The other six treatment groups were fed the basal diet supplemented with 0.5% dandelion leaves (T2), 1% fenugreek seeds (T3), combination of 0.5% dandelion leaves and 1% fenugreek seeds (T4), enzyme treated dandelion leaves 0.5% (T5), enzyme treated fenugreek seeds 1% (T6), and combination of enzyme treated dandelion leaves (0.5%) and (1%) fenugreek seeds (T7). The histomorphological study of liver and small intestines was conducted among different treatment groups.
Results: The results revealed the hepato-protective nature of both dandelion leaves and fenugreek seeds either alone or in combination with or without enzyme treatment when compared with the control group. Moreover, the histomorphological findings of jejunum revealed the beneficial effect of dandelion leaves, fenugreek seeds and enzymes on the intestinal mucosa in terms of cellular infiltration, architecture of villi, villus height/crypt depth ratio, thereby improving the intestinal health.
Conclusion: The dandelion leaves and fenugreek seeds have hepato-protective nature and beneficial effect on the intestinal morphology particularly when included along with enzymes in the diet of broiler chicken.
Keywords: broiler chicken, dandelion, fenugreek, histomorphology.

Impact of heat stress on health and performance of dairy animals: A review

Review (Published online: 12-03-2016)
7. Impact of heat stress on health and performance of dairy animals: A review - Ramendra Das, Lalrengpuii Sailo, Nishant Verma, Pranay Bharti, Jnyanashree Saikia, Imtiwati and Rakesh Kumar
Veterinary World, 9(3): 260-268



   doi: 10.14202/vetworld.2016.260-268



Sustainability in livestock production system is largely affected by climate change. An imbalance between metabolic heat production inside the animal body and its dissipation to the surroundings results to heat stress (HS) under high air temperature and humid climates. The foremost reaction of animals under thermal weather is increases in respiration rate, rectal temperature and heart rate. It directly affect feed intake thereby, reduces growth rate, milk yield, reproductive performance, and even death in extreme cases. Dairy breeds are typically more sensitive to HS than meat breeds, and higher producing animals are, furthermore, susceptible since they generates more metabolic heat. HS suppresses the immune and endocrine system thereby enhances susceptibility of an animal to various diseases. Hence, sustainable dairy farming remains a vast challenge in these changing climatic conditions globally.
Keywords: amelioration, health, heat stress, production, reproduction.

Thursday 10 March 2016

Effect of pre-partum prilled fat supplementation on feed intake, energy balance and milk production in Murrah buffaloes

Research (Published online: 11-03-2016)
6. Effect of pre-partum prilled fat supplementation on feed intake, energy balance and milk production in Murrah buffaloes - Shikha Sharma, Mahendra Singh, Ashwani Kumar Roy and Sunita Thakur
Veterinary World, 9(3): 256-259



   doi: 10.14202/vetworld.2016.256-259



Aim: To investigate the effect of pre-partum prilled fat feeding on dry matter intake (DMI), energy balance and milk production in Murrah buffaloes.
Materials and Methods: Advance pregnant Murrah buffaloes were either received a dietary supplement of prilled fat at 100 g/day for 35 days pre-partum and at 150 g/day for 95 days post-partum (supplemented group [SG]) or did not receive fat supplement (control group [CG]). DMI and the yields of milk and milk component were measured. A body condition score (BCS) was recorded. Energy balance and gross feed efficiency (GFE) were calculated. DMI and BCS were recorded and milk yield (MY), fat, protein, lactose, solid not fat, energy balance were measured. The fat corrected milk yield was calculated.
Results: The DMI was non-significant between groups and periods of study. BCS of buffaloes improved in the SG than CG (p<0.01). The energy intake in terms of total digestible nutrients (TDN%), TDN intake, digestible energy (DE), metabolizable energy/kg of milk, DE of milk, net energy, and GFE were higher (p<0.01) in SG during post-partum period. Crude protein intake was statistically similar in both the groups. MY was higher (p<0.01) in SG than in CG during 95 days of early lactation. Milk fat, fat corrected MY was higher (p<0.01) in SG however protein, lactose and solid not fat content did not varied between the groups. The feed efficiency of the SG was higher (p<0.01) than the CG during the post-partum period.
Conclusion: It was inferred that prilled fat supplementation augments energy balance and milk production in transition Murrah buffaloes.
Keywords: body condition score, buffaloes, dry matter intake, energy balance, milk yield, prilled fat.

Monday 7 March 2016

Serum muscle-derived enzymes response during show jumping competition in horse

Research (Published online: 08-03-2016)
5. Serum muscle-derived enzymes response during show jumping competition in horse - Anna Assenza, Simona Marafioti, Fulvio Congiu, Claudia Giannetto, Francesco Fazio, Daniele Bruschetta and Giuseppe Piccione
Veterinary World, 9(3): 251-255



   doi: 10.14202/vetworld.2016.251-255



Aim: The effect of two jumping competitions, performed in two consecutive weekends, on serum creatine phosphokinase (CPK), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH), urea, creatinine (CREA) concentrations were evaluated in 12 healthy jumper horses.
Materials and Methods: Blood sampling was performed before the 1st day of competition (T0), at the end of each show (J1, J2), on the day after the competition (T1); the same sampling plan was followed during the second weekend (J3, Jand T2).
Results: One-way repeated measures analysis of variance showed an increase in CPK at Jand Jrespect to Tand at Jand Jrespect to all other time points (p<0.05). LDH activity showed an increase at Jrespect to T0, at Jrespect to T0, J1, Jand at Jrespect to all other time points (p<0.05). AST values increased at Jand Jrespect to T(p<0.05). A significant increase of CREA was found at J3respect to T0, Tand Jand at Jrespect to all other time points (p<0.05). A decrease in serum urea levels was found at Jrespect to T0, at Jand Jrespect to Tand T1; at Trespect to T(p<0.05). A positive correlation between urea/CPK (p=0.0042, r2=0.030), LDH/CPK (p<0.0001, r2=0.535), CREA/LDH (p<0.0001, r2=0.263), CREA/CPK (p<0.0001, r2=0.496) was observed.
Conclusion: Our results suggest that 5 days recovery period between the two consecutive competition weekends is insufficient to allow muscle recovery and avoid potential additional stress. The findings obtained in this study improve the knowledge about metabolic changes occurring in athlete horse during the competition to identify muscle alterations following show jumping competitions.
Keywords: horse, muscle enzymes, physical exercise, show jumping competition

Effect of dietary supplementation of garlic, ginger and their combination on feed intake, growth performance and economics in commercial broilers

Research (Published online: 08-03-2016)
4. Effect of dietary supplementation of garlic, ginger and their combination on feed intake, growth performance and economics in commercial broilers - V. K. Karangiya, H. H. Savsani, Shrikant Soma Patil, D. D. Garg, K. S. Murthy, N. K. Ribadiya and S. J. Vekariya
Veterinary World, 9(3): 245-250



   doi: 10.14202/vetworld.2016.245-250



Aim: The present study was carried out to evaluate the effect of supplementation of garlic, ginger and their combination in the diets of broiler chickens and assessment in terms of feed intake, growth performance and economics of feeding.
Materials and Methods: A total of 240 1-day-old Cobb-400 broiler chicks were randomly assigned to four dietary treatments each with three replicates of 20 chicks per replicate (n=60). Four experimental diets were formulated in such a way that control diet (T1) contained neither ginger nor garlic. While, birds in group Tand Twere fed with diets containing 1% garlic and ginger, respectively. Diet 4 (Tgroup) contained a combination of 1% of garlic and ginger. The feeding experiment was carried out for 42 days, and different parameters evaluated includes feed intake, weight gain, feed conversion ratio, gut morphometry, and economics of feeding in terms of return over feed cost (ROFC) and European Performance Efficiency Index.
Results: Feed intake of experimental birds in ginger and mixture of garlic and ginger supplemented groups, i.e., Tand Tgroups have significantly (p<0.05) higher feed intake as compared to control. While, feeding of garlic have non-significant effect on feed intake as compared to other groups. A body weight gain (g/bird) was found to be significantly (p<0.05) higher in garlic (Tgroup) and ginger (T3group) supplemented group as compare to control and garlic and ginger mixture supplemented group (Tgroup). Feed conversion ratio was significantly (p<0.05) lower in ginger (Tgroup) supplemented group as compare to other groups. Mean villi length, villi width and cryptal depth were significantly (p<0.05) higher in Tgroup than rest of all three groups, indicating increased absorptive surface area. ROFC was significantly (p<0.05) lower in Tand Tgroups as compare to control. However, it was not significantly different between control and Tgroup.
Conclusion: On the basis of the results of the study, it is concluded that supplementation of garlic improves the performance of broilers when added at the rate of 1% of broiler ration and can be a viable alternative to antibiotic growth promoter in the feeding of broiler chicken.
Keywords: body weight, broiler chickens, feed intake, garlic, ginger.

Friday 4 March 2016

Effect of heat stress on reproductive performances of dairy cattle and buffaloes: A review

Review (Published online: 05-03-2016)
3. Effect of heat stress on reproductive performances of dairy cattle and buffaloes: A review - Soumya Dash, A. K. Chakravarty, Avtar Singh, Arpan Upadhyay, Manvendra Singh and Saleem Yousuf
Veterinary World, 9(3): 235-244



   doi: 10.14202/vetworld.2016.235-244



Heat stress has adverse effects on the reproductive performances of dairy cattle and buffaloes. The dairy sector is a more vulnerable to global warming and climate change. The temperature humidity index (THI) is the widely used index to measure the magnitude of heat stress in animals. The bjective of this paper was to assess the decline in performances of reproductive traits such as service period, conception rate and pregnancy rate of dairy cattle and buffaloes with respect to increase in THI. The review stated that service period in cattle is affected by season of calving for which cows calved in summer had the longest service period. The conception rate and pregnancy rate in dairy cattle were found decreased above THI 72 while a significant decline in reproductive performances of buffaloes was observed above threshold THI 75. The non-heat stress zone (HSZ) (October to March) is favorable for optimum reproductive performance, while fertility is depressed in HSZ (April to September) and critical HSZ (CHSZ) (May and June). Heat stress in animals has been associated with reduced fertility through its deleterious impact on oocyte maturation and early embryo development. The management strategies viz., nutrition modification, environment modification and timed artificial insemination protocol are to be strictly operated to ameliorate the adverse effects of heat stress in cattle and buffaloes during CHSZ to improve their fertility. The identification of genes associated with heat tolerance, its incorporation into breeding program and the inclusion of THI covariate effects in selection index should be targeted for genetic evaluation of dairy animals in the hot climate.
Keywords: buffaloes, cattle, heat stress zone, reproductive traits, temperature humidity index.

Peperomia pellucida leaf extract as immunostimulator in controlling motile aeromonad septicemia due to Aeromonas hydrophila in red hybrid tilapia, Oreochromis spp. farming

Research (Published online: 05-03-2016)
2. Peperomia pellucida leaf extract as immunostimulator in controlling motile aeromonad septicemia due to Aeromonas hydrophila in red hybrid tilapia, Oreochromis spp. farming - S. W. Lee, K. Y. Sim, W. Wendy and A. K. Zulhisyam
Veterinary World, 9(3): 231-234



   doi: 10.14202/vetworld.2016.231-234



Aim: This study was revealed the potential of Peperomia pellucida leaf extract as an immunostimulator agent in controlling motile aeromonad septicemia due to Aeromonas hydrophila in red hybrid tilapia, Oreochromis sp.
Materials and Methods: In the present study, minimum inhibitory concentration (MIC) of P. pellucida leaf extract against A. hydrophilawas determined through two-fold microbroth dilution method. The plant extract was screening for its active compound using a gas chromatograph mass spectrometer, and the effectiveness of P. pellucida leaf extract as an immunostimulator agent was evaluated. The experimental fish were fed with medicated feed at three different concentrations (25 mg/kg, PP-25; 50 mg/kg, PP-50; and 100 mg/kg, PP-100) of P. pellucida leaf extract for 1 week before they were intraperitoneally exposed to A. hydrophila. Enzyme-linked immunosorbent assay was carried out to determine the value of antibody response to A. hydrophila in fish from a group of fish that received medicated feed, and the percentage of total cumulative mortality of the experimental fish were observed at the end of the experiment.
Results: The results showed that the major bioactive compound is phytol (40%), and the MIC value was 31.5 mg/L. The value of antibody response to A. hydrophila in fish from a group of fish which received medicated feed (PP-25, 0.128±0.014 optical density [OD]; PP-50, 0.132±0.003 OD; and PP-100, 0.171±0.02 OD) was found significantly higher (p<0.05) compared to fish did not receive medicated feed (0.00 OD). Whereas, percentage cumulative mortality of fish from all groups of fish received medicated feed (PP-25, 18.0±3.2%; PP-50, 18.2±2.8%; and PP-100, 17.7±1.8%) were found significantly lower (p<0.05) compared to a group of fish did not receive medicated feed (83.2±1.4%).
Conclusion: The findings of the present study indicated the huge potential of P. pellucida leaf extract as natural immunostimulator agent for aquaculture uses.
Keywords: Aeromonas hydrophila, immunostimulator, motile aeromonad septicemia, red hybrid tilapia.