Friday 12 August 2016

Toxoplasma infection in sheep from south of Iran monitored by serological and molecular methods; risk assessment to meat consumers

Research (Published online: 13-08-2016)
9. Toxoplasma infection in sheep from south of Iran monitored by serological and molecular methods; risk assessment to meat consumers - Belal Armand, Kavous Solhjoo, Manoochehr Shabani-Kordshooli, Mohammad Hasan Davami and Mehdi Sadeghi
Veterinary World, 9(8): 850-855



   doi: 10.14202/vetworld.2016.850-855



Aim: Toxoplasma gondii has a clinical and veterinary importance as it is known to cause congenital disease and abortion both in humans and livestock. Since the contaminated lamb is one of the sources of human infection, this study was performed to determine the prevalence of T. gondii in sheep in south of Iran.
Materials and Methods: Sera and tissue samples (diaphragm and heart) were collected from 370 sheep from slaughterhouse of Jahrom. The samples were taken from both sexes and from 6 to 60 months age. Specific immunoglobulin G antibodies to T. gondii were examined with enzyme-linked immunosorbent assay, and B1 gene nested-polymerase chain reaction detection was done to survey the tissue samples.
Results: The total prevalence of Toxoplasma infection among sheep was found to be 35.94% and 34.32% based on serological and molecular method, respectively. According to serologic and molecular findings, the females were more positive than males forToxoplasma; maximum frequency of positive samples was observed in 24-36 months and the positive samples had been collected more in spring than in summer, but no statistical correlation was observed between prevalence rate and the age and sex of animals or season of sampling.
Conclusion: T. gondii is widely distributed in sheep in Jahrom with a rate comparable with other parts of Iran and the world. It suggested a widespread exposure of sheep in this region to T. gondii. Thus, consumption of undercooked or raw meat presents the transmission risk of the parasite and this might be considered as an important public health problem, mainly for high-risk groups such as the pregnant and the immunodeficient.
Keywords: B1 gene, enzyme-linked immunosorbent assay, meat consumers, nested-polymerase chain reaction, sheep, Toxoplasma gondii.

Transabdominal color doppler ultrasonography: A relevant approach for assessment of effects of uterine torsion in buffaloes

Research (Published online: 13-08-2016)
8. Transabdominal color doppler ultrasonography: A relevant approach for assessment of effects of uterine torsion in buffaloes - Devender, Ramesh Kumar Chandolia, Anand Kumar Pandey, Vishal Yadav, Parveen Kumar and Jasmer Dalal
Veterinary World, 9(8): 842-849



   doi: 10.14202/vetworld.2016.842-849



Aim: The present study was conducted on advanced pregnant buffaloes suffering from uterine torsion to assess the status of fetus and uterus by transabdominal ultrasonography, and the findings were compared with normal advanced pregnant buffaloes.
Materials and Methods: The study was conducted on 20 clinical cases of uterine torsion and 20 normal advanced pregnant buffaloes (control group). The lower ventral area just lateral to linea alba (on both sides of the udder) in standing animals was scanned transabdominally by the two-dimensional convex transducer for various ultrasonographic findings. The data collected were statistically analyzed by “one-way ANOVA” and “independent sample t-test” using computerized SPSS 16.0 software program.
Results: Transabdominal ultrasonography revealed dead fetus in 95% uterine torsion cases and proved useful in imaging internal structures of fetuses while no dead fetus was reported in the control group. Size of umbilicus was found significantly decreased (p<0.05) in uterine torsion group in comparison to control animals, but the decrease in placentomal area was marginal (p>0.05) in uterine torsion group. Average thickness of the uterine wall and mean pixel values of fetal fluids (echogenicity) were found significantly increased (p<0.05) in uterine torsion affected buffaloes in comparison to control group.
Conclusion: Status of fetus (whether live or dead), internal status of uterus, and its contents could be determined by transabdominal ultrasonography in uterine torsion cases and thus determining the prognosis of the uterine torsion cases before going for further manipulations. This will also help in taking all the precautions to avoid death of the fetus.
Keywords: buffaloes, pixel value, transabdominal, ultrasonography, uterine torsion.

Wednesday 10 August 2016

Rumen-protected choline: A significance effect on dairy cattle nutrition

Review (Published online: 11-08-2016)
7. Rumen-protected choline: A significance effect on dairy cattle nutrition - G. Jayaprakash, M. Sathiyabarathi, M. Arokia Robert and T. Tamilmani
Veterinary World, 9(8): 837-841



   doi: 10.14202/vetworld.2016.837-841



Choline is a vitamin-like substance it has multi-function in animal production, reproduction, and health. The transition period is most crucial stage in lactation cycle of dairy cows due to its association with negative hormonal and energy balances. Unfortunately, unprotected choline easily degrades in the rumen; therefore, choline added to the diet in a rumenprotected form. The use of rumen-protected choline (RPC) is a preventive measurement for the fatty liver syndrome and ketosis; may improve milk production as well as milk composition and reproduction parameters. This review summarizes the effectiveness of RPC on animal production, health, and reproduction.
Keywords: choline, dairy cows, health, production, reproduction.

Body weight, egg production, and egg quality traits of gray, brown, and white varieties of Japanese quail (Coturnix coturnix japonica) in coastal climatic condition of Odisha

Research (Published online: 10-08-2016)
6. Body weight, egg production, and egg quality traits of gray, brown, and white varieties of Japanese quail (Coturnix coturnix japonica) in coastal climatic condition of Odisha - Jessy Bagh, B. Panigrahi, N. Panda, C. R. Pradhan, B. K. Mallik, B. Majhi and S. S. Rout
Veterinary World, 9(8): 832-836


   doi: 10.14202/vetworld.2016.832-836



Aim: The present study was conducted to evaluate the performance of gray, brown, and white varieties of Japanese quail (Coturnix coturnix japonica) with respect to body weight, egg production, and egg quality traits in the coastal climatic condition of Odisha.
Materials and Methods: A total of 500-day-old straight run Japanese quail chicks of three varieties, viz., gray, brown, and white were randomly selected and reared in deep litter system at Central Poultry Development Organization, Eastern Region, Bhubaneswar. The weekly body weight of the birds was recorded till their egg production stage (up to 6 weeks of age). The average egg production was recorded every biweekly from 6th to 20th week. Exterior and interior quality of eggs from each variety was determined at 6 weeks of age.
Results: The initial average weekly body weight of three varieties did not differ (p>0.05) among the varieties. However, from 1st to 6th week significantly higher body weight was observed in gray than white and brown. Brown varieties had reached 50% egg production 1 week earlier than gray and white. Brown had higher peak hen day (HD) production or henhoused egg production followed by white and gray. External quality such as: Egg weight, egg length, egg width, volume, shape index, shell weight, shell thickness depicted no significant difference among the varieties except circumference length and circumference width, which were significantly higher (p≤0.05) in gray varieties than brown varieties. Internal egg characteristics such as: Albumen length, albumen width, albumen height, albumen index, yolk length, yolk width, yolk height, yolk index, albumen weight, yolk weight, Haugh unit revealed no significance difference among the varieties.
Conclusion: It may be summarized from the findings that gray excelled in body weight followed by white and brown. Egg production potential in terms of hen house egg production or HD egg production was higher for brown followed by white and gray in the coastal climatic condition of Odisha.
Keywords: body weight, egg production potential, egg quality traits, quail.

Sunday 7 August 2016

Epidemiological and immunopathological studies on Porcine parvovirus infection in Punjab

Research (Published online: 08-08-2016)
5. Epidemiological and immunopathological studies on Porcine parvovirus infection in Punjab - Amninder Kaur, V. Mahajan, G. D. Leishangthem, N. D. Singh, Payal Bhat, H. S. Banga and G. Filia
Veterinary World, 9(8): 827-831



   doi: 10.14202/vetworld.2016.827-831



Aim: The aim of this study was to get the first-hand knowledge about the seroprevalence of Porcine parvovirus (PPV) in Punjab and a diagnosis of PPV from abortion cases of swine using gross, histopathological, and immunohistopathological techniques to observe the tissue tropism of the virus strain.
Materials and Methods: Tissue samples from the reproductive tract of pig (n=32), placental tissue (n=10), and aborted fetuses (n=18) were collected from Postmortem Hall of the Department of Veterinary Pathology, GADVASU, field outbreaks and from butcher houses in and around Ludhiana. These samples were processed for histopathological and immunohistochemical (IHC) studies. For seroprevalence study, 90 serum samples of different sex and age were collected from 15 swine farms of Punjab and were subjected to indirect enzyme linked immunosorbent assay using commercial kit.
Results: Overall, seroprevalence of PPV was found to be 41.1%. Sex and age related difference in the prevalence was noted. In abortion cases grossly congested and emphysematous lungs, congested internal organs with fluid in abdominal cavity and congestion in brain, changes were noted in fetuses, while diffuse hemorrhages and edema was observed in placental tissue. Histopathologically, the most frequent fetal lesions in aborted fetuses were noted in lungs, liver, and brain. IHC staining revealed PPV antigens in sections of heart, liver, lung, spleen, brain, lymph node of fetuses, placenta, and uterus of sow. Gross, histopathological, and IHC examination of the samples confirmed 5 fetus, 2 placenta and 3 female reproductive samples positive for parvovirus infection.
Conclusions: Seroprevalence results may serve as a support either in prevention or control of the disease. IHC is the sensitive technique for diagnosis of PPV associated with the reproductive tract of swine and was found to supplement the gross and histopathological alterations, respectively, associated with the disease.
Keywords: abortion, enzyme-linked immunosorbent assay, histopathology, immunohistochemistry, indirect, Porcine parvovirus, swine.

Friday 5 August 2016

Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests

Research (Published online: 17-07-2016)
11. Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests - H. K. Sharma, S. K. Kotwal, D. K. Singh, M. A. Malik, Arvind Kumar, Rajagunalan and M. Singh
Veterinary World, 9(7): 742-746



   doi: 10.14202/vetworld.2016.742-746



Aim: Brucellosis is a disease of zoonotic importance as it affects both human as well as animal’s health, and therefore, directly affects animal productivity and human efficiency. Therefore, a study was conducted to estimate the seroprevalence of brucellosis in humans in Jammu and surrounding areas.
Materials and Methods: A total of 121 sera samples from humans occupied with professional related to animals were collected and tested for anti-Brucella antibodies by Rose Bengal plate test (RBPT), modified RBPT (mRBPT), standard tube agglutination test (STAT), and indirect enzyme-linked immunosorbent assay (I-ELISA). Sampling was done keeping in view with the occupation, sex, and age.
Results: The overall seroprevalence of brucellosis recorded was 4.96%. The test-wise seroprevalence was 9.91% by RBPT, 9.91% by mRBPT, 9.09% by STAT, and 16.52% by I-ELISA. The prevalence of brucellosis was higher in >35-50 years age group compared to >20-35 years and >50-65 years. Sex-wise seroprevalence was higher in males than females. Taking I-ELISA as standard, the relative sensitivities of mRBPT, RBPT, and I-ELISA were in the order of mRBPT=RBPT>STAT. All the tests revealed high specificity values; however, among different serological tests, I-ELISA detected a maximum number of positive sera samples.
Conclusions: The prevalence of brucellosis was found to be approximately 5%. The adult (>35-50 years) age male group was most vulnerable. The routine diagnosis of brucellosis involved the conventional serological tests, viz., RBPT and STAT, but each was associated with drawbacks which could give either false-positive or false-negative interpretation. Therefore, it is always recommended to use a battery of tests in the diagnosis of brucellosis.
Keywords: brucellosis, humans, indirect enzyme-linked immunosorbent assay, Rose Bengal plate test, seroprevalence, standard tube agglutination test.

Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests

Research (Published online: 17-07-2016)
11. Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests - H. K. Sharma, S. K. Kotwal, D. K. Singh, M. A. Malik, Arvind Kumar, Rajagunalan and M. Singh
Veterinary World, 9(7): 742-746



   doi: 10.14202/vetworld.2016.742-746



Aim: Brucellosis is a disease of zoonotic importance as it affects both human as well as animal’s health, and therefore, directly affects animal productivity and human efficiency. Therefore, a study was conducted to estimate the seroprevalence of brucellosis in humans in Jammu and surrounding areas.
Materials and Methods: A total of 121 sera samples from humans occupied with professional related to animals were collected and tested for anti-Brucella antibodies by Rose Bengal plate test (RBPT), modified RBPT (mRBPT), standard tube agglutination test (STAT), and indirect enzyme-linked immunosorbent assay (I-ELISA). Sampling was done keeping in view with the occupation, sex, and age.
Results: The overall seroprevalence of brucellosis recorded was 4.96%. The test-wise seroprevalence was 9.91% by RBPT, 9.91% by mRBPT, 9.09% by STAT, and 16.52% by I-ELISA. The prevalence of brucellosis was higher in >35-50 years age group compared to >20-35 years and >50-65 years. Sex-wise seroprevalence was higher in males than females. Taking I-ELISA as standard, the relative sensitivities of mRBPT, RBPT, and I-ELISA were in the order of mRBPT=RBPT>STAT. All the tests revealed high specificity values; however, among different serological tests, I-ELISA detected a maximum number of positive sera samples.
Conclusions: The prevalence of brucellosis was found to be approximately 5%. The adult (>35-50 years) age male group was most vulnerable. The routine diagnosis of brucellosis involved the conventional serological tests, viz., RBPT and STAT, but each was associated with drawbacks which could give either false-positive or false-negative interpretation. Therefore, it is always recommended to use a battery of tests in the diagnosis of brucellosis.
Keywords: brucellosis, humans, indirect enzyme-linked immunosorbent assay, Rose Bengal plate test, seroprevalence, standard tube agglutination test.