Biochemical analysis of some serum trace elements in donkeys and horses in Eastern region of Kingdom of Saudi Arabia

Research (Published online: 25-10-2017)

17. Biochemical analysis of some serum trace elements in donkeys and horses in Eastern region of Kingdom of Saudi Arabia

Turke Shawaf, Faisal Almathen, Ahmad Meligy, Wael El-Deeb and Shahab Al-Bulushi

Veterinary World, 10(10): 1269-1274

ABSTRACT

Aim: Little is known about the serum levels of trace elements in donkeys and horses in Saudi Arabia. This study aimed to investigate the levels of some trace elements in these two species in the eastern region of Saudi Arabia and to compare the obtained results with the reference values.

Materials and Methods: Seventeen Arabian horses and twenty eight Hassawi donkeys were randomly selected for this study. All of the studied healthy animals were kept under a uniform feeding protocol without any minerals supplementations. Atomic absorption spectrometer is used to estimate the serum concentrations of selenium (Se), manganese (Mn), chromium (Cr), copper (Cu), iron (Fe), and zinc (Zn) in the analyzed samples.

Results: Significant differences between horses and donkeys were observed in three of the studied elements (Se, Mn, and Cr). Statistically significant differences were found in serum Se and Cr between male and female horses. The male donkey showed higher Mn, Cu, and Zn levels than female animals.

Conclusion: The obtained results of trace element levels in serum of Hassawi donkeys and Arabian horses in Saudi Arabia are considered as the first values to be published for these breeds. When compared to other animals, the measured amounts of Se, Mn, Cr, Cu, Fe, and Zn in the serum of horses and donkey are often differed, possibly because of the varying living or feeding conditions. Moreover, there were some differences in some of the trace elements concentrations related to animal's gender and species (horses and donkeys), which will be considered in the interpretation of the laboratory data.

Keywords: donkey, horse, serum, trace elements.

Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran

Research (Published online: 24-10-2017)

16. Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran

Farhad Sharafati Chaleshtori, Navid Mazroii Arani, Esmat Aghadavod, Afshin Naseri and Reza Sharafati Chaleshtori

Veterinary World, 10(10): 1264-1268

ABSTRACT

Aim: Shiga toxigenic Escherichia coli (STEC) strains as emerging groups of foodborne pathogens are responsible for most foodborne illnesses. The aim of this study was to determine the antibiotic resistance pattern in STEC isolated from traditional milk products and their molecular characterization.

Materials and Methods: A total of 116 samples were randomly purchased from local markets in Kashan, Iran, and evaluated for the occurrence of STEC by culturing and molecular methods. The antibiotic resistance of obtained isolates was determined by Kirby Bauer method. Furthermore, isolates were assayed for the presence of Shiga toxins (stx1 and stx2) and intimin gene (eae).

Results: The incidence of E. coli in 60 ice cream, 30 yoghurt, and 26 cheese samples was 8.33%, 10%, and 11.54%, respectively. The findings showed that 11 out of 11 (100%) E. coli had both stx1 and stx2 while eae gene was not found in E. coli isolated of traditional milk products. For E. coli strains carrying stx1 and stx2, highest antibiotic sensitive levels were related to trimethoprim/sulfamethoxazole, norfloxacin, chloramphenicol, and ciprofloxacin, respectively.

Conclusion: The results showed relationship between the presence of virulence factors and antimicrobial resistance. These results can be used for further studies on STEC as an emerging foodborne pathogen.

Keywords: Escherichia coli, milk products, molecular characterization.

Occurrence, treatment protocols, and outcomes of colic in horses within Nairobi County, Kenya

Research (Published online: 23-10-2017)

15. Occurrence, treatment protocols, and outcomes of colic in horses within Nairobi County, Kenya

Anderson Gitari, James Nguhiu, Vijay Varma and Eddy Mogoa

Veterinary World, 10(10): 1255-1263

ABSTRACT

Aim: The aim of this study was to determine the treatments and their outcomes in horses with colic in Nairobi County, Kenya.

Materials and Methods: This is a retrospective study to determine the occurrence, treatments, pain management, and outcomes of colic in horses in Nairobi County. Association between pain management protocols and the outcomes of colic with regard to recovery or death was also determined. Data collected from four equine practitioners were organized manually and given numerical codes as appropriate to facilitate entry into the computer. The coded data were entered into Microsoft Excel 2010 and exported to StatPlus pro 5.9.8 statistical package for analysis. Simple association tests were done between various factors and occurrence of colic.

Results: The incidence of colic for the 11 years was 3.1%, which constituted 68.0% spasmodic colic, 27.8% impaction colic, and 4.2% displacement colic. Flunixin meglumine as a nonsteroidal anti-inflammatory drug (NSAID) was used as the only pain management treatment in 85.3% of the cases, flunixin meglumine and butorphanol as NSAID-OPIOD combination in 6.4% of the cases, while buscopan as an antispasmodic was recorded in 5.9% of the cases mainly in spasmodic colic. Univariate analysis revealed simple association between various factors and the type of colic a horse was having. There was an association between the type of colic and the decision-making on the pain management protocol to use, whether single analgesic protocol (χ²=22.5, p<0.001) or use of analgesic combinations (χ²=18.3, p<0.001). The type of colic strongly influenced the decision for performing nasogastric intubation (χ²=265, p<0.001), but performing nasogastric intubation was weakly (χ²=4.9, p=0.03) associated with horse recovery from colic. Type of colic also strongly influenced the need for the use of metabolic stimulants, particularly vitamin B-complex (χ²=99.3, p<0.001). Recovery or death of the horse from colic was strongly associated with the type of colic (χ²=250, p<0.001). The possibility of recurrence of colic was weakly (χ²=4.6, p=0.04) determined by the type of colic, a horse had. Multivariate logistic regression revealed that the main cause of death was intestinal displacement and the majority of the horses with intestinal displacement died (β-estimate 2.7, odds ratio=0.07, p=0.007) compared to horses that had impaction colic.

Conclusion: The incidence of colic is 3.1%, and the most common type of colic is spasmodic followed by impaction. The most common pain management protocol for colic is NSAIDs, mainly flunixin meglumine, followed by flunixin-butorphanol combination. Surgery for horses with colic in Nairobi County is not commonly done due to impeding poor prognoses. The horse owners tend to prefer euthanasia for such cases.

Keywords: pain management, colic, incidence, Nairobi, treatment.

Factors influencing growth hormone levels of Bali cattle in Bali, Nusa Penida, and Sumbawa Islands, Indonesia

Research (Published online: 19-10-2017)

14. Factors influencing growth hormone levels of Bali cattle in Bali, Nusa Penida, and Sumbawa Islands, Indonesia

N. K. Suwiti, I N. K. Besung and G. N. Mahardika

Veterinary World, 10(10): 1250-1254

ABSTRACT

Aim: Bali cattle (Bos javanicus) are an Indonesian's native cattle breed that distributed in Asia to Australia. The scientific literature on these cattle is scarce. The growth hormone (GH) of Bali cattle is investigated from three separated islands, namely, Bali, Nusa Penida, and Sumbawa.

Materials and Methods: Forty plasma samples were collected from each island, and the GH was measured using a commercial enzyme-linked immunosorbent assay kit. The data were analyzed based on the origin, sex, and cattle raising practices.

Results: We found that the GH level (bovine GH [BGH]) of animal kept in stall 1.72±0.70 μg/ml was higher than free-grazing animal 1.27±0.81 μg/ml. The GH level was lower in female (1.22±0.62 μg/ml) compared to male animals (1.77±0.83 μg/ml).

Conclusion: We conclude that the level of BGH in Bali cattle was low and statistically equal from all origins. The different level was related to sex and management practices. Further validation is needed through observing the growth rate following BGH administration and discovering the inbreeding coefficient of the animal in Indonesia.

Keywords: Bali cattle, growth hormone, Indonesia.

Epidemiology of bovine fascioliasis in the Nile Delta region of Egypt: Its prevalence, evaluation of risk factors, and its economic significance

Research (Published online: 18-10-2017)

13. Epidemiology of bovine fascioliasis in the Nile Delta region of Egypt: Its prevalence, evaluation of risk factors, and its economic significance

Abdelgawad S. El-Tahawy, Eman K. Bazh and Reda E. Khalafalla

Veterinary World, 10(10): 1241-1249

ABSTRACT

Aim: This study focuses on the risk factors associated with the prevalence of Fasciola affecting cattle population in three provinces belonging to the Nile Delta of Egypt and to estimate the economic losses as a result of fascioliasis.

Materials and Methods: From January 2015 to end of December 2015, records of 21 farms (4976 cattle) were analyzed to screen the prevalence of fascioliasis among cattle farms, to identify its associated risk factors and its economic impacts on Nile Delta region of Egypt.

Results: The overall prevalence of fascioliasis in the Nile Delta region of Egypt was 9.77%. The prevalence of fascioliasis was found to be statistically significantly associated with age, sex, breed, and type of farms. The highest prevalence was observed in <2 age group (10.91%), and the lowest was >3 age groups (8.35%). In terms of body condition scores, cattle with medium and poor conditions were associated with fascioliasis more than those with good body condition. Besides, cattle raised in organic farms were associated with lower risk of fascioliasis than those in conventional farms. The prevalence of fascioliasis was noted more prominent in districts with moderate temperatures and with relative humidity (>60%). The annual overall costs for fascioliasis were estimated to be 221.2 USD/cow due to the significant reduction in body weight, reduction in milk production, and the treatment costs for fascioliasis.

Conclusion: The results provided could be helpful for improving the control and preventive strategies.

Keywords: cattle, Egypt, fascioliasis, Nile Delta, prevalence, risk factors.

Organizational leadership perspectives in implementation of the One Health approach: A case of the Zoonotic Disease Unit and core One Health implementers in Kenya

Research (Published online: 17-10-2017)

10. Organizational leadership perspectives in implementation of the One Health approach: A case of the Zoonotic Disease Unit and core One Health implementers in Kenya - Thomas Manyibe Nyariki, Mathew Muturi, Athman Mwatondo, Michael Cheruiyot, Harry Oyas, Vincent Obanda, Francis Gakuya, Rees Murithi Mbabu and Mercy Muthoni Mugambi

International Journal of One Health, 3: 57-65

  doi: 10.14202/IJOH.2017.57-65

Abstract

Aim:
This study examined organizational leadership as a cross-sectoral collaboration factor in the implementation of the One Health (OH) approach using Kenya’s Zoonotic Disease Unit and its core OH implementers as an example.

Materials and Method:
The study used a mixed methods research design. A semi-structured questionnaire was administered to 71 respondents, and key informants were interviewed using an interview guide. All the seven key informants and 53 (74%) of the respondents participated in the study. Data were checked for consistency, coded, entered into the Statistical Package for the Social Sciences, and analyzed using descriptive and correlational statistics. Interview data were transcribed and analyzed thematically.

Results:
From the analysis, 41/53 (77.2%) of the respondents were senior personnel, 51/53 (85%) of them had worked for 5 years and above in their organizations, and 38/53 (71.7%) had at least a Master’s degree. The study established that although most leaders in the organizations had embraced the OH approach, they were not actively involved in its implementation due to constraints such as inadequate funding. There were moderate and statistically significant positive correlations between participation in leadership roles in implementing the OH approach and the level of awareness (Rs (51)=0.54, p<0.001) as well as level of sensitization (Rs (51)=0.52, p<0.001). Majority (86%) of respondents acknowledged that top government leaders were not well sensitized about the OH approach.

Conclusion:
Organizational leadership plays an important role in the implementation of the OH approach. However, sensitization of leaders about the OH approach needs to be enhanced and expanded.

Keywords:
cross-sectoral collaboration, One Health approach, organizational leadership, sensitization.

Occurrence of virulent and antibiotic-resistant Shiga toxin-producing Escherichia coli in some food products and human stool in Egypt

Research (Published online: 15-10-2017)

12. Occurrence of virulent and antibiotic-resistant Shiga toxin-producing Escherichia coli in some food products and human stool in Egypt

Osman Mohamed Hamed, Maha Ahmed Sabry, Nawal A. Hassanain, Eman Hamza, Ahmed G. Hegazi and Marwa Badawy Salman

Veterinary World, 10(10): 1233-1240

ABSTRACT

Aim: Shiga toxin-producing Escherichia coli (STEC) represent a severe public health issue worldwide, causing life-threatening diseases in the human gastrointestinal tract. This study aimed to determine the occurrence of virulent and antibiotic-resistant STEC in retail meat and milk products and human stool samples and to characterize the genes encoding for virulence and antibiotic resistance among the identified STEC isolates.

Materials and Methods: A total of 260 food samples were randomly collected from retail markets in different localities of El Giza Governorate, Egypt. 50 stool specimens were obtained from children that had diarrhea at Embaba Fever Hospital. All collected samples were initially subjected to bacteriological examination and serotyping, and then subsequently, the isolates were exposed to polymerase chain reaction application and sequencing for the identification of the virulence-related genes. Finally, the virulent STEC isolates were tested for antibiotic susceptibility.

Results: Serotyping of the 76 biochemically identified isolates showed that 18 were STEC with a predominance of non- O157 (16) while 2 O157:K-serotype was detected only in one food and one human isolate. Molecular identification of the virulence genes illustrated that the minced meat showed the highest prevalence of STEC (8%) as compared to the other food products. In the humans, the O157 was the only serotype that expresses the Shiga toxin-associated gene (eaeA). Antibiotic susceptibility test displayed that 13 of the 17 food and human isolates (76.47%) were resistant to cephalothin (KF30). 9 of the 13 cephalothin-resistant isolates harbor the β lactamase (blaTEM)-resistant gene. All isolates were sensitive to chloramphenicol, ciprofloxacin, amikacin, and gentamicin. DNA sequencing and phylogenetic analysis of the stx2-positive minced meat isolate revealed a high genetic relatedness with beef minced meat from the USA and Australia.

Conclusion: This study showed the predominance of non-O157 among the identified isolates. Minced meat showed the highest prevalence of STEC as compared to the other food products, and this work illustrates the necessity to consider the food products as a potential source of the non-O157 STEC serotypes. DNA sequencing and phylogenetic analysis revealed a high genetic relatedness with beef minced meat from the USA and Australia. This highlights the high probability of worldwide spread of such serotypes, signifying the importance of the one world concept.

Keywords: antibiotic resistance bacteria, cheese, DNA sequencing, human stool, meat, polymerase chain reaction, serotyping, shiga toxin-producing Escherichia coli.